亚洲精品一区二区三区不卡,美国3级大鸡巴www.jb,二零二五a国产网站,日本中文高清视频一区

產(chǎn)品中心您現(xiàn)在的位置：首頁(yè) > 產(chǎn)品展示 > > 特色試劑盒 > Direct cAMP ELISA kit

Direct cAMP ELISA kit

更新時(shí)間：2022-01-28

簡(jiǎn)要描述：

Direct cAMP ELISA kit
Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification.

型號(hào)：廠商性質(zhì)：代理商瀏覽量：818

  • Highly sensitive with an optional acetylation protocol which increases sensitivity >10-fold

  • High throughput format, results for up to 39 samples in duplicate in just 3 hours

  • Flexible assay, species independent

  • Easy-to-use pre-coated plate and liquid color-coded reagents reduce errors

  • Efficient and well-established sample handling protocols

  • Widely cited in peer-reviewed publications

This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.
<strong>Direct cAMP ELISA kit</strong> Standard curve - Acetylated
Typical standard curve used to calculate cAMP concentrations for Acetylated assay format.
Please mouse over
<strong>Direct cAMP ELISA kit</strong> Standard curve - AcetylatedADI-900-066A <strong>Direct cAMP ELISA kit</strong> Standard curve - Non-Acetylated







Product Details

Alternative Name:cyclic AMP

Sensitivity:Non-acetylated: 1.18 pmol/ml (range 0.78 - 200 pmol/ml)
Acetylated: 0.006 pmol/ml (range 0.078 - 20 pmol/ml)

Assay Time:3 hours

Applications:ELISA, Colorimetric detection

Application Notes:For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include tissue culture.

Wavelength:405 nm

Species reactivity:Species independent

Crossreactivity:cyclic AMP (100%)
AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%)

Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.

Shipping:Shipped on Blue Ice

Contents:GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine

Scientific Background:Cyclic AMP (cAMP) is one of the most important “second messengers" involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

Regulatory Status:RUO - Research Use Only

Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal


Product Literature References

Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity: M. Laudette, et al.; Cell Death Dis. 12, 4149 (2021), Abstract;
Exendin-4 stimulates autophagy in pancreatic β-cells via the RAPGEF/EPAC-Ca2+-PPP3/calcineurin-TFEB axis: F.P. Zummo, et al.; Autophagy (2021), Abstract;
Exhaustive endurance exercise activates brain glycogen breakdown and lactate production more than insulin-induced hypoglycemia: T. Matsui, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 320, 500 (2021), Abstract;
Functional evidence for biased inhibition of G protein signaling by YM-254890 in human coronary artery endothelial cells: Q. Peng, et al. ; Eur. J. Pharmacol. 891, 173706 (2021), Application(s): Intra-cellular cAMP level with human coronary artery endothelial cells (HCAEC), Abstract;
Glucagon transiently stimulates mTORC1 by activation of an EPAC/Rap1 signaling axis: S. Sunilkumar, et al.; Cell. Signal. 84, 110010 (2021), Abstract;
Inhibition of PDE4 by apremilast attenuates skin fibrosis through directly suppressing activation of M1 and T cells: Q. Lu, et al.; Acta Pharmacol. Sinica (2021), Abstract;
Pre-clinical evaluation of dual targeting of the GPCRs CaSR and V2R as therapeutic strategy for autosomal dominant polycystic kidney disease: A. Di Mise, et al.; FASEB J. 35, 21874 (2021), Abstract;
Rescue of cell surface expression and signalling of mutant follicle-stimulating hormone receptors: H. Sharika, et al.; Endocrinology (2021), Abstract;
The level of secondary messengers and the redox state of NAD+/NADH are associated with sperm quality in infertility: S.N. Galimov, et al.; J. Reprod. Immunol. 148, 103383 (2021), Abstract;


留言框

  • 產(chǎn)品：

  • 您的單位：

  • 您的姓名：

  • 聯(lián)系電話：

  • 常用郵箱：

  • 省份：

  • 詳細(xì)地址：

  • 補(bǔ)充說(shuō)明：

  • 驗(yàn)證碼：

    請(qǐng)輸入計(jì)算結(jié)果（填寫阿拉伯?dāng)?shù)字），如：三加四=7

抽插抽查视频| 天天无码| 一本道综合一区| 亚洲一二三四五不卡免费视频| 91免费亚洲一区| 日韩操美女| 大香蕉精品伊人| 久精品网站| 中文字幕有码在线视频播放| 黑人亚洲女人视频| 深夜老司机视频提供者| 俄罗斯国产一级射精视频免费观看| 青青草wwwwww| 啊啊啊啊啊日逼视频| 操鸡巴大鸡巴| 日韩AV小电影| 使劲操逼啊啊啊视频| 91丝袜美腿中文字幕视频在线| 大鸡吧操死我网站。The| 熟女少妇视频性一区二区三区| 日韩后入美女| 中文字幕在线一二三四成人| 欧美少妇猛烈进入| 蜜色AV| 福贡县| 中文字母av在线| Av在饯一二三四| 疏勒县| 国产精品被大鸡巴狂插骚逼| 亚洲高清巨臀后入| 最新韩国日本性爱视频| 红桃影视av| 大香蕉社区一区二区| 看美女被日逼视频播放| 少妇口爆一区| 大鸡巴日逼网站| 日韩毛片一级无码观看久久久久久| 摸逼视频日本| 韩国美女日B视频| 久久中文区红桃| 大BBBB日韩视频|