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ENZO熱銷產(chǎn)品——LTB4 ELISA kit,白三烯B4檢測(cè)試劑盒

更新時(shí)間:2025-01-23   點(diǎn)擊次數(shù):309次

白三烯(Leukotriene, LT)是花生四烯酸的5-脂氧合酶代謝的主要產(chǎn)物,能夠刺激白細(xì)胞的功能,包括溶酶體酶的釋放、粘附和多形核白細(xì)胞(即嗜中性、嗜堿性和嗜酸性白細(xì)胞)的聚集,被認(rèn)為是炎癥性疾病和免疫調(diào)節(jié)的強(qiáng)效介質(zhì)。半胱氨酰白三烯(LTC4、LTD4和LTE4)是通過(guò)將半胱氨酸衍生物加到LTA4上形成的,LTC4合酶將LTA4轉(zhuǎn)化為L(zhǎng)TC4限制了半胱氨酰白三烯的形成速率。半胱氨酰白三烯作為炎癥中的強(qiáng)效脂質(zhì)介質(zhì),可誘導(dǎo)平滑肌收縮和毛細(xì)血管通透性增加。

Enzo Life Sciences的高品質(zhì)熱銷產(chǎn)品LTB4 ELISA kit(貨號(hào):ADI-900-068)具有超高靈敏度和高文獻(xiàn)引用率,批次間差異小,上市多年廣受用戶好評(píng)。

 

產(chǎn)品亮點(diǎn):

● 超靈敏度,對(duì)LTB4的超靈敏測(cè)量,檢測(cè)濃度低至5.63 pg/mL;

● 高文獻(xiàn)引用,在眾多同行評(píng)審的出版物中被廣泛引用;

● 寬動(dòng)態(tài)范圍,適用于多種樣本類型;

● 高通量格式,做復(fù)孔的情況下,可在4小時(shí)內(nèi)獲得多達(dá)39份樣本的結(jié)果;

● 批間批內(nèi)差異小,檢測(cè)結(jié)果具有高度的可重復(fù)性;

● 完-全定量結(jié)果,優(yōu)于WB的半定量分析。

 

產(chǎn)品參數(shù):

產(chǎn)品名稱

LTB4 ELISA kit

別名

Leukotriene B4 ELISA kit

產(chǎn)品貨號(hào)

ADI-900-068 / ADI-901-068

規(guī)格

1*96 wells/ 5*96 wells

應(yīng)用

Colorimetric detection, ELISA

應(yīng)用筆記

For the quantitative determination of LTB4 in culture supernatants, 

plasma, saliva, and urine from any species. Cited sample types 

include bronchioalveolar lavage fluid, cell lysate, crevicular fluid, 

tissue, serum and whole blood.

分析時(shí)長(zhǎng)

4 hours

產(chǎn)品組分

GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash 

buffer concentrate, Standard, pNpp Substrate, Stop solution

靈敏度

5.63 pg/ml

檢測(cè)范圍

11.7 - 3,000 pg/ml

反應(yīng)物種

Species independent

檢測(cè)波長(zhǎng)

405 nm

使用/穩(wěn)定性

Store all components at +4°C, except conjugate at -20°C.

運(yùn)輸條件

Blue Ice

 

高分文獻(xiàn)引用:

近期,Adams發(fā)表了題為《Leukotriene A4 hydrolase inhibition improves age-related cognitive decline via modulation of synaptic function》的文章,在該項(xiàng)研究中,Adams等人使用了Enzo的LTB4 ELISA試劑盒檢測(cè)小鼠血漿,測(cè)定了老年小鼠、給藥白三烯A4水解酶抑制劑(LTA4H)的小鼠體內(nèi)炎癥介質(zhì)白三烯B4(LTB4)水平,證明了LTA4H抑制劑可以減少LTB4的產(chǎn)生,并改善小鼠與年齡相關(guān)的認(rèn)知能力下降。

LTB4 ELISA.jpg

 

(A) Plasma LTB4 measured from unstimulated blood is significantly increased from 3 to 22.5 months (M). Unpaired t test, **P = 0.0011, F = 2.952 (n = 15 and 11). (B) Plasma LTB4 levels from calcimycin-stimulated blood are significantly reduced following 4 weeks of dosing with LTA4H inhibitors AKST1220 or SC57461A. Kruskal-Wallis test, P = 0.0006 followed by Dunn’s multiple comparisons: vehicle versus AKST1220, **P = 0.0034; vehicle versus SC57461A, *P = 0.0212; and AKST1220 versus SC57461A, P > 0.9999 (n = 5, 6, and 9).

 

Adams團(tuán)隊(duì)對(duì)LTB4 ELISA kit的真實(shí)評(píng)價(jià):

Q: Why did you use Enzo’s LTB4 ELISA Kit?

A: Species-independent measurement of LTB4 in culture supernatant, plasma, saliva, urine, and other cited sample types.

Q: How do you position Enzo’s LTB4 ELISA Kit?

A: The LTB4 ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. It is designed to assay up to 39 samples in duplicate with a broad dynamic range that is suitable for a wide variety of sample matrices, including bronchioalveolar lavage fluid, cell lysate, crevicular fluid, tissue, serum and whole blood.

 

LTB4 ELISA kit最新引用文獻(xiàn):

1.  Leukotriene A4 hydrolase inhibition improves agerelated cognitive decline via modulation of synaptic function: J.M. Adams, et al.; Sci. Adv. 9, eadf8764 (2023), Abstract

2. Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract — Full Text

3.  Neobavaisoflavone inhibits allergic inflammatory responses by suppressing mast cell activation: R. Liang, et al.; Int. Immunopharmacol. 110, 108953 (2022), Abstract

4. Effect of Dysglycemia on Urinary Lipid Mediator Profiles in Persons With Pulmonary Tuberculosis: M.B. Arriaga, et al.; Front. Immunol. 13, 919802 (2022), Abstract

 

Enzo Life Sciences總部設(shè)于美國(guó)紐約,在研發(fā)、生產(chǎn)、銷售生物研究試劑方面擁有40多年的成功經(jīng)驗(yàn),可提供多種試劑盒、蛋白、抗體、多肽、小分子、標(biāo)簽探針染料等產(chǎn)品,用于研究靶標(biāo)鑒定及驗(yàn)證、高通量分析、基因表達(dá)分析、蛋白質(zhì)檢測(cè)及細(xì)胞分析等相關(guān)領(lǐng)域。

 


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