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Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

更新時間:2023-12-21   點擊次數(shù):481次

EpiCypher是一家為表觀遺傳學和染色質生物學研究提供高質量試劑和工具的專業(yè)制造商。EpiCypher生產的在E. coli中表達的重組人單核小體(組蛋白H2A、H2B、H3和H4各2個;accession numbers:H2A-P04908;H2B-O60814;H3.1-P68431;H4-P62805)由601位處的147個DNA堿基對纏繞在組蛋白上構成。組蛋白H2B(由專門的半合成方法產生)在120位處含有泛素賴氨酸。核小體是染色質的基本單位,Lowary和Widom鑒定的601序列是一個含有147堿基對的序列,對組蛋白八聚體具有高親和力,可用于核小體組裝,并含有5'biotin-TEG基團。適合用作酶篩選分析或效應蛋白結合實驗的底物。

[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715


產品詳情

標簽: Biotinylated

保存溫度:Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

產品形式: H2BK120ub dNuc (14.4 µg protein weight, 25 µg DNA + protein) in 22.5 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 5.12 μM. MW = 216,985.9 Da.


驗證數(shù)據(jù)

Epicypher熱銷產品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

Figure 1: Western blot data

Western Analysis of Nucleosome, Recombinant Human, H2BK120ub. 

Top Panel: Unmodified H3 (Lane 1) and H2BK120ub containing nucleosomes 

(Lane 2) were probed with an anti-H2BK120ub antibody and analyzed via ECL

 readout. Only the H2BK120ub sample produced a detectable signal. Bottom 

Panel: Detail from Coomassie stained gel showing unmodified nucleosomes 

(Lane 1) and H2BK120ub nucleosomes (Lane 2).

Epicypher熱銷產品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

Figure 2: Mass spec data H2BK120ub protein analyzed by high 

resolution mass spectrometry. Expected mass = 22,321.8 Da. Determined 

mass = 22,320.3 Da.

Epicypher熱銷產品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

Figure 3: Protein gel data

Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant 

Human, H2BK120ub (1 μg) to demonstrate the purity of the histones in the 

preparation. Sizes of molecular weight markers and positions of the core 

histones (H2A, H2BK120ub, H3.1, and H4) are indicated.

Epicypher熱銷產品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

Figure 4: DNA gel data

Nucleosome, Recombinant Human, H2BK120ub dNucs resolved via native 

PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free 

DNA (100 ng). Lane 2: Intact H2BK120ub nucleosome (400 ng).


訂購詳情

貨號

產品名稱

規(guī)格

16-0396   

Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated   

25 µg   



Epicypher熱銷產品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多詳細信息或相關產品,請聯(lián)系EpiCypher中國授權代理商-欣博盛生物 

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